CellTour · a mode
FlipTheScale
Every instrument has a smallest detail it can separate, its resolution. Below that, two points blur into one. For each thing, pick the simplest instrument that can still resolve it.
Honest notes
- Each limit is a best case. A real picture is often blurrier: a stained cell in a light microscope, or a frozen sample in an electron microscope, rarely reaches the limit.
- Seeing something is not the same as resolving it. You can spot a star or a single hair; you cannot resolve its width.
- Sizes are typical and rounded; real things vary.
Sources
- Sizes: the TenTimes ladder (Milo and Phillips, Cell Biology by the Numbers, and textbook values); a hair: Ley 1999, The Physics Factbook; an α-helix turn: Pauling, Corey and Branson 1951.
- The eye: James Madison University, Resolution and Size Primer.
- The light microscope: Nikon MicroscopyU, Resolution.
- The electron microscope with biological samples: Faas FGA et al. (2012). Virtual nanoscopy. J Cell Biol 198:457–469. doi:10.1083/jcb.201201140
- Atomic models: Nakane T et al. (2020). Single-particle cryo-EM at atomic resolution. Nature 587:152–156. doi:10.1038/s41586-020-2829-0